Sombers Lab

Single Cell Amperometry

  •  An electrode is positioned on top of a single cell to create an artificial synapse.

  • The potential at the electrode is held at 800 mV, and the passage of current monitored.

  • Upon exocytosis, dopamine and other catecholamines (including norepinephrine, epinephrine) are oxidized, yielding characteristic peaks when plotting current vs. time.

  • Each peak represents a single vesicle fusing to the cell membrane.